Composite

Part:BBa_K1615081:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)


Linker+dCBD fused to TVEL5 driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2653
    Illegal BglII site found at 2814
    Illegal BamHI site found at 2877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2459


Design Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.


Source

TVEL5 Laccase from Tramates versicolor. dCBD from Trichoderma reesei. Lac expression cassette from iGEM10_Washington.

References